why use nucleic acid detection reagents

Recent advances and perspectives of nucleic acid detection ...

With the advance in molecular biology technology, nucleic acid detection methods have developed rapidly and become a revolutionary technology for virus detection. Especially, the method based on polymerase chain reaction (PCR) is characterized by rapid detection, high sensitivity and specificity, which has been regarded as the "gold standard" for virus detection.

Author Minzhe Shen, Ying Zhou, Jiawei Ye, Abdu Ahmed Abdullah AL-maskri, Yu Kang, Su Zeng, Sheng Cai

Efficacy Assessment of Nucleic Acid Decontamination ...

Jul 13, 2016 · Nucleic acid was extracted and amplified by real-time (RT-) PCR. Negative effects of the different decontamination reagents regarding the inhibition-free nucleic acid extraction and real-time (RT-) PCR amplification were excluded by integration of …

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Intro to Detection Methods Used in Molecular Biology ...

Chemiluminescence detection of nucleic acids. The above protocol may also be used if chemiluminescence reagents are being used for nucleic acid detection on Southern and northern blots.

Nucleic Acid Extraction Methods - BioChain Institute Inc.

Devices. There are numerous devices that are used in nucleic acid extraction methods, these are Spin Columns, Beads or Magnetic Beads, Automated Nucleic Acid Extraction Systems or Liquid Handling Robots as well as Lab-on-a-Chip Cartridges and Microfluidics.

WHO Dos and Don’ts for molecular testing

Jan 31, 2018 · Molecular detection methods have the ability to produce a large volume of nucleic acid through the amplification of trace quantities found in samples. While this is beneficial for enabling sensitive detection, it also introduces the possibility of contamination through the spreading of amplicon aerosols in the laboratory environment.

Detection of nucleic acid modifications by chemical reagents

To understand the biological role of these modified nucleosides, nucleic acid scientists need tools to specifically label, detect and enrich modified nucleic acids. These tools comprise a diverse set of chemical reagents which have been established in the early years of nucleic acid research.

Labeling Oligonucleotides and Nucleic Acids—Section 8.2 ...

The ULS technology is based on the use of a platinum dye reagent that forms a stable adduct with the N-7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, peptide–nucleic acid conjugates (PNA) and oligonucleotides (Figure 8.2.10). In protein contexts, ULS reagents are reactive with cysteine residues and other thiols.

Overview of Influenza Testing Methods CDC

Overview

Sensitive detection of viral nucleic acids using the ...

Nucleic Acid Isolation Kit is designed to extract viral nucleic acid from a variety of sample types with a range of viral loads. • The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit provides a sensitive and simple method for nucleic acid extraction from virus-containing samples, as demonstrated by successful detection of as few as 50 copies ...

File Size 471KB

Quantitative PCR (qPCR) Biocompare

Oct 18, 2018 · Real-Time PCR, also referred to as Quantitative PCR (or qPCR), was developed as a precise, efficient and rapid method for nucleic acid detection. This technique is based on traditional Polymerase Chain Reaction (PCR) technology with a few improvements Real-Time PCR combines nucleic acid amplification and detection in one step.

Nucleic Acid Stains—Section 8.1 Thermo Fisher Scientific ...

PicoGreen, OliGreen and RiboGreen quantitation reagents and their Quant-iT reagent counterparts in Nucleic Acid Quantitation in Solution—Section 8.3 set a benchmark for the detection and quantitation of DNA, oligonucleotides and RNA in solution. These reagents offer extremely simple and rapid protocols as well as linear ranges that span up to ...

Nucleic Acids DNA extraction and Dische’s Diphenylamine ...

Suspend the spooled DNA in 3 ml of distilled water. MIX. Add to tubes 2 ml of DNA solution; 1 ml of DNA solution with 1 ml H 2 O; 2 ml of H 2 O; Add 2 ml of the Dische’s diphenylamine reagent to each tube and mix thoroughly. Place in a boiling water bath for 10 minutes. Evaluate your results. A clear tube indicates no nucleic acids.

Are Coronavirus Tests Accurate? - MedicineNet Health News

He was hospitalized, testing negative for coronavirus several times before eventually receiving a positive result. On Jan. 30 the doctor posted "Today nucleic acid testing came back with a positive result, the dust has settled, finally diagnosed," according to the BBC. Dr. Wenliang passed away on February 7 in Wuhan, the epicenter of the outbreak.

Reagents and kits - Integrated DNA Technologies

DNase- and RNase-free solutions ideal for resuspending and diluting nucleic acids. Each lot is tested using RNaseAlert ® and DNaseAlert™ reagents to ensure there is no detectable nuclease activity. Individual lots are also screened for endotoxins using a Limulis amebocyte lysate (LAL) assay.

Blocking Reagent For nucleic acid hybridization and ...

The Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids. It has also been used as a blocking agent in immunohistochemistry.

Instructions for PerkinElmer® New Coronavirus Nucleic Acid ...

Coronavirus Nucleic Acid Detection Kit . v 1.0 . For prescription use only. For in vitro diagnostic use only. ... 2. Use the reagents within 30 days once opened. 3. Completely thaw the reagents ...

Technical Guide for Non-Radioactive Nucleic Acid …

In recent years, non-radioactive nucleic acid labeling and detection method-ologies have become available in response to a desire by researchers and their institutions to move away from the use of radioisotopes. Advancements made in the areas of chemiluminescence and fluorescence have allowed for an easier transition.

File Size 946KB

New Coronavirus (2019-nCoV) Nucleic Acid Detection Kit ...

sensitive nucleic acid based-detection of sars-cov-2 authorized under fda emergency use authorization To address growing supply constraints worldwide, PerkinElmer has expanded the production capacity of its extraction and RT-PCR tests at its global facilities and has the capability to ship millions of these solutions to its customers.

Stains, labels and detection strategies for nucleic acids ...

requirements for nucleic acid detection, and‘uorescent methods currently dominate in many of these applications. Ann Clin Biochem 2002;39:114-129 Introduction The range of assay formats for nucleic acids, oligo-(deoxy)nucleotides (oligos), polymerase chain reac-tion (PCR) products and related molecules is exten-sive.

Massively multiplexed nucleic acid detection using Cas13 ...

Apr 29, 2020 · In the CARMEN platform, nanoliter droplets containing CRISPR-based nucleic acid detection reagents 7 self-organize in a microwell array 8 to …

Assessment of eight nucleic acid amplification ...

Nucleic acid amplification technologies (NAATs) are high-performance tools for rapidly and accurately detecting infectious agents. They are widely used in high-income countries to diagnose disease and improve patient care. The complexities associated with test methods, reagents, equipment, quality control and assurance require dedicated laboratories with trained staff, which can exclude their ...

Author Jason L. Cantera, Heather White, Maureen H. Diaz, Shivani G. Beall, Jonas M. Winchell, Lorraine Lill...

cobas SARS-CoV-2

cobas® SARS-CoV-2 for use on the cobas® 6800/8800 Systems is a real-time RT-PCR test intended for the qualitative detection of nucleic acids from SARS-CoV-2 in nasopharyngeal and oropharyngeal swab samples from patients with signs and symptoms suggestive of COVID-19 (e.g., fever and/or symptoms of acute respiratory illness).

PerkinElmer SARS-CoV-2 Nucleic Acid Detection Kit RUO ...

Sensitive & Specific Nucleic Acid-based Detection of 2019-nCoV ORF1ab and N Genes. The PerkinElmer ® SARS-CoV-2 Nucleic Acid Detection kit (RUO) has a limit of detection 20 copies/mL for the SARS-CoV-2 ORF1ab and N genes. It does not show cross reactivity with common respiratory tract pathogens and blood stream pathogens including:

Covid-2019 nucleic acid testing reagents

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TransGen Biotech Co., LTD

Transgen Biotech Co., LTD was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Now TransGen is a leading manufacturer of more than 200 molecular and cellular biology reagents.

Solved Alcohol Is Considered To Be A Significant Reagent ...

Why is this so? Expert Answer Alcohol is considered to be a significant reagent for the isolation for nucleic acid because in neutral aqueous solutions, the negatively charged p view the full answer

XABT donates 2019-nCoV nucleic acid detection kits to Italy

Mar 26, 2020 · BEIJING, March 25, 2020 /PRNewswire/ -- On March 19, the 2019-nCoV Detection Kit and Nucleic Acid Extraction Donation & Shipment Ceremony was held in Beijing.Beijing Applied Biological Technologies Co., Ltd. (XABT) was invited to the event at which the biologics maker donated an allotment of nucleic acid detection kits that it had produced to Italy.

Labeling of Nucleic Acids - Vector Laboratories, Inc.

Shorter strands of nucleic acids such as oligonucleotides, PCR primers, or capture probes used to identify nucleic acid binding proteins are specifically and efficiently labeled at either the 5’ or the 3’ end using the 5’ E’ ndTag™ or the 3’ E’ ndTag™ Nucleic Acid Labeling Systems, respectively.

Nucleic Acid Detection Technologies — Labels, Strategies ...

The range and scope of nucleic acid-based assays continues to expand (e.g., medical, forensic, and environmental applications) ()()().Many of the types of nucleic acid assays developed require a secondary detection technology, e.g., a label, because a nucleic acid does not have intrinsic properties that are useful for direct high-sensitivity detection.

Nucleic Acid Detection Using CRISPR/Cas Biosensing ...

For infectious diseases, rapid and accurate identification of the pathogen is critical for effective management and treatment, but diagnosis remains challenging, particularly in resource-limited areas. Methods that accurately detect pathogen nucleic acids can provide robust, accurate, rapid, and ultrasensitive technologies for point-of-care diagnosis of pathogens, and thus yield information ...

Instructions for PerkinElmer® New Coronavirus Nucleic Acid ...

Use the reagents within 30 days once opened. 3. Completely thaw the reagents before use. ... Nucleic Acid Detection Kit (“your product” in the conditions below), the relevant

Why the false-negative rate of nucleic acid tests in new ...

Why the false-negative rate of nucleic acid tests in new crown pneumonia is so high. Experts say virus nucleic acid is not detected due to degradation. Our reporter Zhang Ye. Since the outbreak of New Coronary Pneumonia, the topic of excessive false-negative nucleic acid detection has been the focus of attention of all parties.

Assessment of eight nucleic acid amplification ...

Nucleic acid amplification technologies (NAATs) are high-performance tools for rapidly and accurately detecting infectious agents. They are widely used in high-income countries to diagnose disease and improve patient care. The complexities associated with test methods, reagents, equipment, quality control and assurance require dedicated laboratories with trained staff, which can exclude their ...

Labeling of Nucleic Acids - Vector Laboratories, Inc.

Shorter strands of nucleic acids such as oligonucleotides, PCR primers, or capture probes used to identify nucleic acid binding proteins are specifically and efficiently labeled at either the 5’ or the 3’ end using the 5’ E’ ndTag™ or the 3’ E’ ndTag™ Nucleic Acid Labeling Systems, respectively.

Isothermal Amplification NEB

Isothermal amplification methods provide detection of a nucleic acid target sequence in a streamlined, exponential manner, and are not limited by the constraint of thermal cycling. Although these methods can vary considerably, they all share some features in common.

Detection of Nucleic Acids and Proteins - The Cell - NCBI ...

The advent of molecular cloning has enabled the isolation and characterization of individual genes from eukaryotic cells. Understanding the role of genes within cells, however, requires analysis of the intracellular organization and expression of individual genes and their encoded proteins. In this section, the basic procedures currently available for detection of specific nucleic acids and ...

Nucleic Acid Test - an overview ScienceDirect Topics

Dongyou Liu, in Molecular Medical Microbiology (Second Edition), 2015. Nucleic Acid Tests. Nucleic acid tests such as polymerase chain reaction (PCR) are new-generation technologies that have higher sensitivity than blood cultures and better specificity than serologic tests. Useful targets include insertion sequences (IS 6501 or IS711), the BCSP31 gene (coding for a 31-kDa immunogenic outer ...

LabCorp COVID-19 RT-PCR test EUA Summary

reaction (rRT-PCR) test for the qualitative detection of nucleic acid from SARS-CoV-2 in upper and lower respiratory specimens (such as nasal, nasopharyngeal or ... Reagent Manufacturer Catalog #

Contamination with Nucleic Acid in Molecular Settings ...

• Use of equipment, reagents and disposals is room specific • Always use high quality of reagents (aliquot in small amount) • Clean all used areas with 10% bleach or NA removing agent before and after use • UV Decontaminate instruments and hoods (ceilings) after use • Use appropriate waste containers . Clinicallabconsulting

CRISPR-Cas12a-assisted nucleic acid detection Cell …

Apr 24, 2018 · Using synthetic biomolecular components, many methods have been developed for fast nucleic acid detection 1,2,3; however, they may not be able to …

QIAamp Circulating Nucleic Acid Kit - QIAGEN Online Shop

Circulating RNA was purified from 4 ml pooled plasma using the QIAamp Circulating Nucleic Acid Kit, with an elution volume of 50 μl. The standard kit protocol was compared with the specialized protocol for miRNA. miRNA 30b yield was quantified using a TaqMan microRNA assay (Applied Biosystems), and miRNA 16 yield was quantified using a Human miScript Assay (QIAGEN).

cobas SARS-CoV-2

cobas® SARS-CoV-2 for use on the cobas® 6800/8800 Systems is a real-time RT-PCR test intended for the qualitative detection of nucleic acids from SARS-CoV-2 in nasopharyngeal and oropharyngeal swab samples from patients with signs and symptoms suggestive of COVID-19 (e.g., fever and/or symptoms of acute respiratory illness).

Kits, reagents and viruses - Virology Down Under

Apr 12, 2020 · Nucleic acid extraction market leaders.[14] One fallout from this pandemic is that labs may look to more broadly diversify their reliance on any given supplier. And another may that companies developed processes to more quickly surge production capacity including ensuring stocks of key reagents needed in those kits.

WO2010001969A1 - Method for amplification of target ...

Disclosed is a mutation detection method which can detect a mutation in a single reaction system with excellent sensitivity and reliability. Primers (Xmt) and (Xwt) are used to amplify a target nucleic acid sequence in which a nucleotide to be detected is mutated with higher amplification efficiency than that for a target nucleic acid sequence in which the nucleotide to be detected is normal.

6 Nucleic Acid Sequence-Based Identification for Detect …

Nucleic Acid Sequence-Based Identification for Detect-to-Warn Applications Culture-based assays, which typically run for 12 to 24 hours or longer, are normally viewed as an unimpeachable standard for the identification (ID) of microbes.

AGUIDE TO NUCLEIC ACID LABELING …

PHOTOPROBE ® Reagentsand theFastTag ® System PHOTOPROBE ®ReagentsandtheFastTag ®Nucleic AcidLabelingSystemsarethemethodsofchoice ...

26 questions with answers in NUCLEIC ACID DETECTION ...

Apr 30, 2020 · DNA Green Viewer™ is a new and safe nucleic acid stain, an alternative to the traditional Ethidium bromide (EB) stain for detecting double-stranded DNA, single-stranded DNA, and RNA in …

Label IT Nucleic Acid Labeling Kits FAQs

Yes. For best results, use purified nucleic acid in labeling reactions. For most applications, it is ideal to use concentrated stocks (~1 mg/ml) of the nucleic acid. Q18. Why should labeled DNA probes be denatured with the Denaturation Reagent D1?

GeneReach Biotechnology Corp.(瑞基海洋生物科技股份有限公 …

POCKIT™ combo combines nucleic acid extraction and detection into one package and comes in as a transportable hard-shell suitcase including a taco™ mini Automatic Nucleic Acid Extraction System, a POCKIT™ Nucleic Acid Analyzer, a mini-centrifuge, and two micropipettes. Dimension; 629 (W) × 497 (D) × 353 (H) mm / 20 kg; Components

California coronavirus testing hamstrung by shortage of ...

The missing reagent is a homopolymeric nucleic acid used to isolate the virus’ genetic material, or RNA, from the patient’s sample. It is made by the diagnostics testing giant QIAGEN.

Emergency Approval Coronavirus - China Med Device

China has accelerated the Emergency Approvals under the threat of Novel Coronavirus (“2019-nCoV”). National NMPA granted seven Emergency Approvals to Coronavirus Nucleic Acid Detection Reagent manufacturers in January, while provincial NMPA offices paid all-out efforts to help contain the virus.

Coronavirus qPCR test Kits & Rapid Kits COVID-19 available ...

Nucleic Acid Extraction. Fast extraction of nucleic acids; Processing of blood, serum, plasma, urine, CSF, and swabs; One protocol/reagent that fits to all sample types; CE-IVD certification of both instrument and reagent; Flexible loading of 1-6 or 1-12 samples; A starting volume of 200 or 400 μL; Pre-filled consumables to minimize errors

OliGlo™ Nucleic Acid Labeling Kits Your Source for High ...

Choose Marker Gene Technologies, Inc. for high quality OliGlo™ nucleic acid labeling kits, labeling uniquely through the phosphate backbone. Find easy to use kits backed up with excellent customer service and technical support.

SHERLOCK nucleic acid detection with CRISPR nucleases ...

Sep 23, 2019 · Rapid detection of nucleic acids is integral to applications in clinical diagnostics and biotechnology. We have recently established a CRISPR-based diagnostic platform that combines nucleic acid ...

DNA/RNA Extraction Reagent - ViralXpress 3095

DNA/RNA Extraction Reagent - ViralXpress Overview The Light Diagnostic ViralXpress™ nucleic acid extraction reagent is intended for the purification of viral DNA and RNA from plasma, serum, cerebral spinal fluid, amniotic fluid, tissue, bone marrow, infected cells or cell culture supernatants.

Summary of NMPA approved novel coronavirus 2019-nCoV …

Intended use In vitro qualitative detection of Anti-2019-nCoV antibody from specimen. It is only intended for the supplementary indicator for suspected cases of negative 2019-nCoV nucleic acid detection, or combination with nucleic acid detection in the diagnosis of suspected cases.

After Wuhan trials, China to mass produce rapid test kits ...

Until now, China’s National Medical Products Administration has approved 10 new coronavirus nucleic acid detection reagents and six antibody detection reagents. world Updated Mar 12, 2020 02:50 IST

Aptima HPV Assay - Hologic

Aptima HPV Assay 2 AW-12820 Rev. 001 Aptima® General Information – Tigris DTS System and Panther System Intended Use The Aptima HPV assay is an in vitro nucleic acid amplification test for the qualitative detection of E6/E7 viral messenger RNA (mRNA) from 14 high-risk types of human papillomavirus (HPV) in cervical specimens.